Fragmentation of bacteriophage S13 replicative form DNA by restriction endonucleases from Hemophilus influenzae and Hemophilus aegyptius

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Fragmentation of bacteriophage S13 replicative form DNA by restriction endonucleases from Hemophilus influenzae and Hemophilus aegyptius.

The restriction enzymes Hind from Hemophilus influenzae and Hae,,, from Hemophilus aegyptius cleave bacteriophage S13 replicative form (RF) DNA into 13 and 10 specific fragments, respectively. The sizes of these fragments were estimated by gel efectrophoresis, electron microscopy, and pyrimidine isostich analysis. The Hind and Hae,,, fragments were ordered relative to each other by cross digest...

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Specific cleavage of simian virus 40 DNA by restriction endonuclease of Hemophilus influenzae.

A bacterial restriction endonuclease has been used to produce specific fragments of SV40 DNA. Digestion of DNA from plaque-purified stocks of SV40 with the restriction endonuclease from Hemophilus influenzae gave 11 fragments resolvable by polyacrylamide gel electrophoresis, eight of which were equimolar with the original DNA. The fragments ranged from about 6.5 x 10(5) to 7.4 x 10(4) daltons, ...

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Induction of Streptomycin Resistance in Sensitive Hemophilus Influenzae by Extracts Containing Desoxyribonucleic Acid from Resistant Hemophilus Influenzae

Resistance to streptomycin, of a degree exceeding 1000 microg. per ml., has been induced in sensitive strains of Hemophilus influenzae by exposure for 10 minutes to desoxyribonucleic acid-containing extracts isolated from a strain of type b Hemophilus influenzae which had emerged resistant to 1000 microg. of streptomycin per ml. DNA is essential for the process which brings out this change; the...

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The Extraction of Hemagglutinin from Strains of Hemophilus Aegyptius

A serologically active substance was extracted from cells of Hemophilus aegyptius with dilute NAOH. This substance directly hemagglutinated human erythrocytes. The activity of this material was demonstrated at a near neutral pH and was precipitated at acid pH. Preliminary biochemical determination showed this serologically active material to be predominately polysaccharide. Davis, Pittman, and ...

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Hemophilus influenzae type b pneumonia.

SUMMARY Thirteen patients with Haemophilus influenzae type b pneumonia are reported to emphasise the clinical, radiographic, and therapeutic aspects of this illness. All but one patient was tnder 21 years of age. The presenting complaint was a variable duration of upper respiratory infection and fever in most cases. One-third of patients had radiographic evidence of pleural involvement ; one-th...

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ژورنال

عنوان ژورنال: Virology

سال: 1976

ISSN: 0042-6822

DOI: 10.1016/0042-6822(76)90115-x